After uploading code the serial connection failed on macOS. I must use the Arduino IDE to upload code via BOOTSEL but next time using PIO the same problem.
Meanwhile the actual version is 2.2., annoying.
The actual version of what? The Arduino core or the uploader program?
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ArduinoCore-mbed is 2.2.0
After updating to 2.3.1 the problem persists. With the Arduino IDE I have no problems with the serial port after uploading, this happens only with Pio.
Ok, found the problem. Sorry 
Which was what exactly? 
It was a problem with Ethernet3 library, the Ethernet.softreset() function caused a never ending loop and blocked therefore the serial communication.
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